Multiplayer Characters in Story Mode
To get them, beat their level on at least normal difficulty.
Big Tony in Chicago
Hybrid Mutant in Siberia
Jacque de la Morte in Notre Dame
Khallos in Atom Smasher
Machinist in Robot Factory
Ozor Mox in Return To Planet X
Reaper Splitter in Space Station
Sadako in NeoTokyo
Stone Golem in Aztec Ruins
The Colonel in Wild West
(Thanks to eoseth@gmail.com)

Multiplayer Maps
Chasm: Beat Arcade League - Honorary League - Outnumbered but Never Outpunned - Can’t Handle This with at least a Bronze.
Chinese: Beat Arcade League - Honorary Leauge - Elimination Series - Starship Whoopers with at least a Bronze.
Circus: Beat Challenge - Monkeying Around - Dam Bursters with at least a Bronze.
Compound: Beat Spaceship: 2401 on Normal.
Hangar: Beat Arcade League - Amateur League - Beginner’s Series - Top Shot with at least a Bronze.
Nightclub: Beat Arcade League - Amateur League - Too Hot to Handle - Disco Inferno with at least a Bronze.
Robot Factory: Beat Arcade League - Amateur League - It’s a Blast - Demolition Derby with at least a Bronze.
Scrapyard: Beat Arcade League - Amateur League - Mode Madness - Scrap Medal with at least a Bronze.
Site: Beat Story mode on Hard difficulty.
Streets: Beat Spaceship: 2401 on Easy.
Utopia: Beat Arcade League - Honorary League - Maximus - Cold Corpse Caper with at least a Bronze.
(Thanks to eoseth@gmail.com)

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